Tom Horton

Associate Professor, Mycorrhizal Ecology SUNY-College of Environmental Science and Forestry Department of Environmental and Forest Biology 1 Forestry Drive Syracuse, NY 13210 Office phone: 315-470-6794 Fax: 315-470-6934 trhorton@esf.edu Office: 350 Illick Hall Lab: 447 Illick Hall

• Teaching
• Research interests
• Students and visitors
• Publication list

Students interested in joining the lab should contact me via email at: trhorton@esf.edu

NEWS FLASH (Feb. 2010): PhD assistanship available beginning summer/fall 2010.

I am seeking one PhD student for a collaborative project focused on the role of ectomycorrhizal fungi in the invasion of Pinaceae in Argentina. Collaborators are Daniel Simberloff and Martin Nuñez at University of Tennessee. The position is funded through NSF with three years of salary and tuition waiver. The project site is on Isla Victoria on Lake Nahuel Huapi, Argentina's first National Park where a century ago 135+ exotic trees species were planted, including many that are invasive in other locations. Surprisingly, few of these species have invaded the native Nothofagus forest on Isla Victoria. The work follows previous research at the site (PDF of Ecology pub). The current project is designed to investigate how mammals, wind, and belowground growth influence fungal spread away from existing plantations, supporting subsequent invasion by the exotic conifers. Additional research opportunities are available with respect to the ectomycorrhizal communities in the native Nothofagus stands. The student will help in our efforts to quantify dispersal of ectomycorrhizal fungi through wind, animal dispersed spores and mycelial spread. Student's with Master's degree in plant ecology, mycology, soil science, environmental science or other related field and those with experience using a combination of morphological and molecular approaches to identify fungi from ectomycorrhizal roots are encouraged to apply.

Application: Please send (by March 15, 2010) 1) transcripts and/or GRE scores (unofficial copies are OK initially), 2) CV, 3) contact information for 3 references, and 4) a letter of application which (i) describes your interest in the position, (ii) describes your career goals, and (iii) details your work or educational experience that is most relevant to this position.

Send questions and application materials (PDF preferred) to Horton via email at trhorton@esf.edu or via surface mail to Tom Horton, Dept. of EFB, 1 Forestry Drive, SUNY-ESF, Syracuse, NY 13210, USA.

Teaching:

• EFB 320, General Ecology. 3 credits, Fall
  • Lecture page (syllabus)
  • Lab page
• EFB 496/796, Advanced Mycology: Basidiomycetes. 2 credits, Fall 2009
• EFB 428/628, Mycorrhizal Ecology. 3 credits, Spring.
• EFB 797, Mycorrhizal Symbiosis. 1 credit, Spring

• SEMINAR SERIES: Behavior, Ecology, and Evolution Research Seminars is a monthly seminar series designed to bring researchers (faculty/postdocs/grads) from SU and ESF together. Click B.E.E.R.S. for meeting place, times and schedule. All meetings are held at the new SU Life Sciences Building room 106 and begin at 4:00 pm. Come a bit earlier for preseminar treats.
  • Summertime gone, come and gone, my oh my. Check the link above for updates on the complete fall schedule.
  • Next talk: Stephanie Eby (SU), September 21, Title to be announced.

Some useful links

My research interests:

All of my research is applicable to various issues in conservation biology of ectomycorrhizal fungi and plants. Mycorrhizal fungi are primarily below ground, cryptic and essentially considered microbial, leading plant and ecosystem ecologists to largely 'black-box' their roles in plant communities and ecosystem dynamics. A second focus of mine is the development and use of PCR-based techniques to identify fungi directly from mycorrhizal root tips and soil hyphae, thus giving us an ability to peek into the black-box (see Horton and Bruns, 2001). It is amazing to me that most textbooks barely mention mycorrhizal symbioses. Indeed, many ecologists still consider mutualisms as special cases (acacia ants, orchid moths). Part of this bias comes from the fact that models of mutualisms predict that they are unstable and therefore should not be common (there are probably some socio-political issues here as well!!). But four examples of very stable mutualisms should put that misunderstanding to rest: chloroplasts in plant cells, mitochondria in eukaryotic cells, N-fixing bacteria in plant roots, and lichens. Further, around 80-90% of all plants associate with mycorrhizal fungi that are typically mutualistic, so mycorrhizal mutualisms are a fifth case demonstrating the ubiquity of mutualisms in nature. Is the mycorrhizal symbiosis stable? Mycorrhizal fungi have been associated with plants since they colonized land over 400 million years ago.

Current projects: updated February-2010

At the risk of pigeonholing the breadth of their work, my graduate students have investigated or are investigating the role of mycorrhizal fungi in plant community dynamics (Sara Ashkannejhad, Tera Galante, Mikey O'Brien, Yazmin Rivera), restoration ecology (Kris Dulmer, Chris Hazard, Erin Page, Sam Tourtelott), and ecosystem dynamics (Joe Vineis). See a summary of projects below.

• Fungi Along a Natural Nitrogen Availability Gradient - This is an NSF funded project seeking to add a fungal perspective to the PnET model. Our job on this multi-institution, multi-discipline project is to identify fungi from EM root tips, bulk soils, and in-growth core bags. The first year data from root tips suggests nich partitioning along the nitrogen availability gradient by some EM fungi. We are currently analyzing the second year root tip data, and soil and in-growth core bag data for both years. This project is in collaboration with Erik Hobbie and others (Scott Ollinger, Ruth Varner, Serita Frey) at the University of New Hampshire. Joe Vineis (student)
• Population genetics of ectomycorrhizal fungi - We are investigating the population structure of two fungi, Suillus pictus in NY and Pisolithus tinctorius (Puerto Rico). This is work is in collaboration with Kathleen Pitcher and guided by Dr. Annette Kretzer. Yazmin Rivera (student)
• The role of mycorrhizal fungi in plant invasions
  • Isla Victoria in Argentina had a large number of conifer species introduced about 100 years ago. Today, only a handful have become invasive despite many more having life history traits that would suggest they should be invasive. Here we are investigating what is inhibiting conifer invasion on the island. Specifically, is a lack of mycorrhizal fungi in soils (spore and hyphal inoculum) inhibiting invasion of conifers at distances away from the plantations? This project is in collaboration with Dr. Martin Nuñez and Dr. Dan Simberloff at the University of Tennessee. PDF of Ecology pub. Again we ask, is a lack of mycorrhizal fungi in soils (spore and hyphal inoculum) inhibiting invasion of conifers at distances away from the plantations? Yazmin Rivera (student)
  • Carribean pine was introduced to Puerto Rico and is showing evidence of becoming invasive there.
  • Another study investigates the mycorrhizal associations of Epipactis helleborine in New York. This orchid was first recorded in Syracuse in the late 1800s and has since spread across North America. The orchid is associated with ascomycetes, especially truffle species in the genus Tuber. Tuber is a below ground fruiting (hypogeous) fungus best known for the prized edible species in Europe. We have evidence for a number of unidentified Tuber species associated with the orchid in New York, some of which may also have been introduced. Tuber species can be found in a variety of habitats, which may have supported the naturalization and invasion of the orchid across the continent. Dave Muska, Jess Rumburg, Maria Moskalenko (former undergrad students)
• Mycorrhizal status of transgenic American chestnut - Here we ask if transgenic American chestnut lines developed to resist chestnut blight fungus (Cryphonectria parasiticus) will still associate with ectomycorrhizal fungi on the roots. This is a new project and is part of the effort by the American Chestnut Research and Restoration Project in collaboration with Dr. Bill Powell and Dr. Chuck Maynard. Sam Tourtelott (Student)
• Biodiversity and biogeography of ectomycorrhizal fungi in Honduran pine stands - We are conducting a survey of ectomycorrhizal fungi found in native pine stands in Honduras. The stands are close to the southern extent of the genus. The project has many facets including helping the local people harvest and market choice edible fungi in the Boletus edulis group as a low impact economic use of native forests and teaching molecular techniques in a remote field camp setting. This is a new project in collaboration with Operation Wallacea.

Past projects (Gone but not forgotten....send money now!)

• Oregon Dunes - We have good evidence that suilloid fungi are critical for pines establishing away from mycorrhizal networks under primary succession. We are now looking at why other fungi are less important and how far wind disperses spores. Sara Ashkannejhad (former student), Tera Galante (former student)
• The myco-heterotrophic plant Pterospora andromedea (pinedrops) is common in the western United States, but rare in the East. An explanation for this regional
  rarity could be that its specific mycorrhizal symbiont is uncommon in the East. In the western United States several haplotypes of pinedrops exist. These haplotypes are specific to one of two common ectomycorrhizal fungi within the genus Rhizopogon subgenus Amylopogon, both associated with members of Pinaceae. In this study only one eastern pinedrops haplotype/fungal symbiont pair was detected. The plant haplotype was not unique. However, the fungal symbiont is an undescribed species in the subgenus Amylopogon and associates with Pinus strobus. This eastern Rhizopogon species is uncommon in the spore banks of eastern P. strobus forests. The rarity of eastern pinedrops is correlated with the rarity of spores of its fungal symbiont in eastern soils. Chris Hazard (former student)
• Modeling the Ectomycorrhizal Inoculum Potential in Soils - One can often observe relatively pure stands of eastern hemlock adjacent to relatively pure stands of various maple species. This also marks a shift from EM dominated soils to AM dominated soils. Hemlock (EM) seedlings are less likely to establish in the maple (AM) stands. We have established a field bioassay manipulative experiement across this gradient using hemlock and will use the data model the mycorrhizal potential of these soils. This project is funded by the Mianus River Gorge Preserve. Mikey O'Brien (former student)

van der Heijden MGA, Horton TR (accepted) Socialism in soil? The importance of mycorrhizal fungal networks for facilitation in natural ecosystems. Journal of Ecology (Special feature on facilitation in plant communities).

Nuñez MTA, Horton TR, Simberloff D (2009) Lack of belowground mutualisms hinders Pinaceae invasions. Ecology 90:2352-2359.

Horton TR, Arnold AE, Bruns TD (2008) FESIN workshops at ESA - the mycelial network grows. Mycorrhiza 19:283-28

Horton TR, van der Heijden M (2008) The role of symbioses in seedling establishment and survival. In: Seedling Ecology and Evolution. Leck M, Parker VT, Simpson B, Eds. Cambridge University Press.

Bidartondo et al. (2008) Preserving accuracy in GenBank. Science 319: 1616 (This is a letter signed by many.)

Hobbie EA, Horton TR (2007) Evidence that saprotrophic fungi mobilise carbon and mycorrhizal fungi mobilise nitrogen during litter decomposition. New Phytologist 173: 447–449. This was an invited comment on Lindahl et al. (2007) Spatial separation of litter decomposition and mycorrhizal nitrogen uptake in a boreal forest. New Phytologist 173: 611–620.

Horton TR (2006) The number of nuclei in basidiospores of 63 species of ectomycorrhizal Homobasidiomycetes. Mycologia 98: 233-238.

Ashkannejhad S, Horton TR (2006) Ectomycorrhizal ecology under primary succession on coastal sand dunes: interactions involving Pinus contorta, suilloid fungi and deer. New Phytologist 169:345-354.

Becerra A, Zak MR, Horton TR, Micolini J (2005) Ectomycorrhizal and arbuscular mycorrhizal colonization of Alnus acuminata from Calilegua National Park (Argentina). Mycorrhiza 15: 525-531.

Horton TR, Molina R, Hood K (2005) Douglas-fir ectomycorrhizae in 40 and 400 year-old stands: mycobiont availability to late successional western hemlock. Mycorrhiza 15: 393-403.

Fujimura KE, Smith JE, Horton TR, Weber NS, Spatafora JW (2005) Pezizalean mycorrhizas and sporocarps in ponderosa pine (Pinus ponderosa) after prescribed fires in eastern Oregon, USA. Mycorrhiza 15: 79-86.

Nouhra ER, Horton TR, Cazares E, Castellano M (2005) Morphological and molecular characterization of selected Ramaria mycorrhizae. Mycorrhiza 15: 55-59.

Bruns TD, Baar J, Grogan P, Horton TR, Kretzer A, Redecker D, Tan J, Taylor DL (2005) Natural history and community dynamics of ectomycorrhizal fungi following the Mt. Vision fire. pp33-40, In Lessens Learned from the October 1995 Mt. Vision Fire; CD ROM published by Points Reyes National Seashore.

Potente J, Horton T (2004) Tale of a ragged fringe. Long Island Botanical Society Quarterly Newsletter, 13(4): 27-29.

Lilleskov EA, Bruns TD, Horton TR, Taylor DL, Grogan P (2004) Detection of forest stand-level spatial structure in ectomycorrhizal fungal communities. - FEMS Microbiology Ecology 49: 319-332.

Horton TR (2002) Molecular approaches to ectomycorrhizal diversity studies: variation in ITS at a local scale . Plant and Soil 244: 29-39.

Bruns TD, Kretzer AM, Horton TR, Stendel E"Acey-Ducey", Bidartondo MI, Szaro TM (200) Current investigations of fungal ectomycorrhizal communities in the Sierra Nevada forest. USDA Forest Service Gen. Tech. Rep. PSW-GTR: pp. 83-89.

Becerra A, Daniele G, Domínguez L, Nouhra E and Horton T (2002) Ectomycorrhizae between Alnus acuminata H.B.K. and Naucoria escharoides (Fr.:Fr.) Kummer from Argentina. Mycorrhiza: 12:61-66.

Lilleskov EA, Fahey TJ, Horton TR, Lovett GM (2002) Nitrogen deposition and ectomycorrhizal fungal communities: a belowground view from Alaska. Ecology 83: 104 - 115.

Horton, Thomas R. & Bruns, Thomas D (2001)  The molecular revolution in ectomycorrhizal ecology: peeking into the black-box. Molecular Ecology 10 (8): 1855-1871.

Chapela IH, Osher LJ, Horton TR, Henn MR (2001) Ectomycorrhizal fungi introduced with exotic pine plantations induce soil carbon depletion. Soils Biology and Biochemistry 33: 1733-1740.

Baar J, Horton TR, Kretzer A, Bruns TD (1999) Mycorrhizal recolonization of Pinus muricata from resistant propagules after a stand-replacing wildfire . New Phytologist 143: 409-418.

Allen MF, Trappe JM, Horton TR (1999) NATS truffle and truffle-like fungi 8: Rhizopogon mengeisp. nov. (Boletaceae, Basidiomycota). Mycotaxon 70: 149-152.

Stendell ER, Horton TR, Bruns TD (1999) Early effects of prescribed fire on the structure of the ectomycorrhizal fungal community in a Sierra Nevada ponderosa pine forest. Mycological Research 103: 1353-1359.

Horton TR, Bruns TD, and Parker TV (1999) Ectomycorrhizal fungi associated with Arctostaphylos contribute to Pseudotsuga menziesii establishment. Canadian Journal of Botany 77: 93-102.

Horton TR, Bruns TD (1998) Multiple host fungi are the most frequent and abundant ectomycorrhizal types in a mixed stand of Douglas fir (Pseudotsuga menziesii D. Don) and bishop pine (Pinus muricata D. Don). New Phytologist 139(2): 331-339.

Horton TR, Cázares E, Bruns TD (1998) Ectomycorrhizal, vesicular-arbuscular and dark septate fungal colonization of bishop pine (Pinus muricata) seedlings in the first five months of growth after wildfire. Mycorrhiza 8:11-18.

Bruns TD, Szaro TM, Gardes M, Cullings KW, Pan JJ, Taylor DL, Horton TR, Kretzer A, Garbelotto M, Li Y. (1998) A sequence database for the identification of ectomycorrhizal Basidiomycetes by phylogenetic analysis.   Molecular Ecology, v.7, n.3, (1998): 257-272.

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Images on this webpage taken by Dave Pilz, Annette Kretzer, or Tom Horton. General design by Tim Szaro.