Practical: High Performance Liquid Chromatography June 4-8, 2001 Jahn Laboratory, SUNY-ESF Syracuse, New York

Sponsored By—
Faculty of Chemistry Continuing Education, SUNY ESF
Waters Coorporation
New York Sea Grant

This course is an intensive 5-day workshop designed for end users of High Performance Liquid Chromatography. Topics to be covered include:

• Introduction to HPLC theory
• Column selection and maintenance
• Equipment maintenance and repair
• Methods development including detector options
• Sample preparation techniques

Participants will spend the morning sessions in a lecture format covering the basic theory, correct installation and troubleshooting techniques. Afternoons will be devoted to the laboratory where participants will run samples, dissemble and assemble equipment and work on the hands-on aspects of methods development.

Unique Opportunities: The focus of this workshop is for end users to gain practical experience using different HPLC instrumentation under the watchful eye of the instructor. A strong emphasis will be placed on the correct installation and operation of the instrumentation that is essential for those "hard-to do" separations. Working in cooperation with Agilent Technologies and Waters Coorporation, HP 1100, Water's Breeze and Millennium instrument platforms will be available for the laboratory sessions. Detector options utilized will include UV, fluorescent, photodiode array, electrochemical, and mass selective (LCMS). Troubleshooting tips and an extensive working manual detailing the materials from the course will be provided to all participants. The end result will be to gain broad practical experience in a large number of different techniques including methods development, instrument repair and validation.

Instructor: Professor Greg Boyer SUNY-ESF, Faculty of Chemistry, is a natural products biochemist with more than 25 years experience designing HPLC separations. His research interests include analysis of marine and freshwater toxins, drug metabolism and bioactive peptides.

Who Should Attend: This class is designed for the intermediate user, i.e the HPLC operator that has been using HPLC for anywhere from 1-3 years but who would like to use the instrument more efficiently. New operators will also benefit from the sections on instrument setup and operation.

Workshop Location: The workshop will be held in the Jahn Laboratory for Chemical Research at the SUNY College of Environmental Science and Forestry. Directions will be sent with acknowledgement of registration.

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