Publications

Powell, W.A., C.A. Maynard, B. Boyle, and A. Seguin. 200_.  Fungal and bacterial resistance in transgenic trees. In: M. Fladung, Ed., Transgenic Trees.  Springer (in press)

Polin, L. D., H. Liang, R. E. Rothrock, M. Nishii, D. L. Diehl, A. E. Newhouse, C. J. Nairn, W. A. Powell & C. A. Maynard. 2005. Agrobacterium-Mediated Transformation of American Chestnut (Castanea dentata (Marsh.) Borkh.) Somatic Embryos. Plant Cell Tissue and Organ Culture (in press).

Powell, W.A., S.A. Merkle, H. Liang, and C.A. Maynard. 2005. Blight Resistance Technologies: Transgenic Approaches. In, proc. of conf. on restoration of American chestnut to forest lands, Steiner, K.C. and J.E. Carlson (eds.)

Newhouse, A., F. Schrodt, C. Maynard, and W. Powell.  200_.  Agrobacterium transformation of American elm (Ulmus americana) leaf pieces.   In: K. Wang, Ed., Agrobacterium Protocols: (2nd edition) Methods in Molecular Biology Book Series, Humana Press, Inc., Totowa, NJ. (in press)

Maynard, C.A., L. D. Polin, S. LaPierre, R. E. Rothrock, and W. A. Powell. 200_. American chestnut (Castanea dentata (Marsh.) Borkh.) transformation. In: K. Wang, Ed., Agrobacterium Protocols: (2nd edition) Methods in Molecular Biology Book Series, Humana Press, Inc., Totowa, NJ. (in press)

Liang, H., H. Gao, C.A. Maynard, and W.A. Powell. 2005. Expression of a self-processing, putative pathogen resistance-enhancing gene construct in Arabidopsis. Biotech. L. 27:435-442

Connors, B.J., M. Miller, C.A. Maynard, and W.A. Powell. 2002. Cloning and characterization of promoters from American chestnut capable of directing reporter gene expression in transgenic Arabidopsis plants. Plant Science 163:771-781
 
Connors, B.J., N.P. Laun, C.A. Maynard, and W.A. Powell. 2002. Molecular characterization of a gene encoding a cystatin expressed in the stems of American chestnut (Castanea dentata). Planta 215:510-514
 
Liang, H. C.M. Catranis, C.A. Maynard, and W.A. Powell. 2002. Enhanced resistance to the poplar pathogen, Septoria musiva, in hybrid poplar transformed with genes encoding antimicrobial peptides. Biotechnol. Lett. 24(5):383-389
 
Connors, B.J., C.A. Maynard, and W.A. Powell. 2001. Expression sequence tags from stem tissue of American chestnut. Biotechnol. Lett. 23:1407-1411
 
Liang, H., C.A. Maynard, R.D. Allen, W.A. Powell. 2001. Increased Septoria musiva resistance in transgenic hybrid poplar leaves expressing a wheat oxalate oxidase gene. Plant Mol. Biol. 45:619-629
 
Powell, W.A., C.M. Catranis, and C.A. Maynard. 2000. Design of self-processing antimicrobial peptides plant protection. Letters in Applied Microbiology 31:163-168
 
Xing, Z.Z., W.A. Powell, and C.A. Maynard. 1999. Development and germination of somatic embryos in American chestnut. Plant Cell Tissue and Organ Culture 57:47-55
 
Maynard, C., Z. Xing, S. Bickel, and W. Powell. 1999. Using Genetic Engineering to Help Save the American Chestnut: A Progress Report. Journal of the American Chestnut Foundation, 12 (2):41-56.
 
Powell, W.A. and C.A. Maynard. 1997. Designing small antimicrobial peptides and their encoding genes. pp.165-172. In Micropropagation, Genetic Engineering, and Molecular Biology of Populus , N.B. Klopfenstein, Y.W. Chun, M.-S. Kim, M.R. Ahuja, eds. M.C. Dillon, R.C. Carman, L.G. Eskew, tech. eds. Tech. Rep. RM-GTR-297. Fort Collins, CO: U.S. Department of Agriculture, Forest Service, Rocky Mountain Forest and Range Experiment Station. 310p.
Xing, Z., M.F. Satchwell, W.A. Powell, and C.A. Maynard. 1997. Micropropagation of American chestnut: Increasing rooting rate and preventing shoot-tip necrosis. In Vitro Cellular and Developmental Biology-Plant 33:43-48
 
Kistler, H.C., U. Benny, and W.A. Powell. 1997. Linear mitochondrial plasmids of Fusarium oxysporum contain genes with sequence similarity to a reverse transcriptase from Neurospora spp. Applied and Environmental Microbiology 63:3311-3313
Powell, W.A., C.M. Catranis, and C.A. Maynard. 1995. Synthetic Antimicrobial Peptide Design. Molecular Plant-Microbe Interactions 8:792-794
 
Powell, W.A. 1995. Vegetative incompatibility and mycelial death of Cryphonectria parasitica monitored with pH indicators. Mycologia 87:738-741
Powell, W.A. and Z.H. Yan. 1995. Recombination between Fusarium oxysporum telomeres and pUC ampicillin resistance gene in a transforming vector. Fungal Genetics Newsletter 42:62-64
 
Rizwana, R. and W.A. Powell. 1995. Ultraviolet light-induced heterokaryon formation and parasexuality in Cryphonectria parasitica. Experimental Mycology 19:48-60
Rizwana, R. and W.A. Powell. 1992. Ultraviolet light-induced instability of vegetative compatibility groups of Cryphonectria parasitica. Phytopathology 82:1206-1211
 
Milgroom, M.G., S.E. Lipari, and W.A. Powell. 1992. DNA fingerprinting and analysis of population structure in the chestnut blight fungus, Cryphonectria parasitica. Genetics 131:297-306
Powell, W.A. and H.C. Kistler. 1990. In vivo rearrangement of foreign DNA by Fusarium oxysporum produces linear self-replicating plasmids. J. Bact. 172:3163-3171
 
Gobbi, E., Y. Wang, R.M. Martin, W.A. Powell, and N.K. Van Alfen. 1990. Mitochondrial DNA of Cryphonectria parasitica: lack of migration between vegetatively compatible strains. Molecular Plant-Microbe Interactions 3:66-71
Powell, W.A. and N.K. Van Alfen. 1987. Differential accumulation of poly(A)+ RNA between virulent and dsRNA-induced hypovirulent strains of Cryphonectria (Endothia) parasitica. Mol. Cell. Biol. 7:3688-3693
 
Powell, W.A. and N.K. Van Alfen. 1987. Two non-homologous viruses of Cryphonectria (Endothia)parasitica reduced the accumulation of specific virulence associated polypeptides. J. Bact. 169:5324-5326
Hansen, D.R., N.K. Van Alfen, K. Gillis, and W.A. Powell. 1985. Naked dsRNA associated withhypovirulence of Endothia parasitica is packaged in fungal vesicles. J. Gen. Virol. 66:2605-2614
Merkle, S.A., Andrade, G.M., Nairn, C.J., Powell, W.A. and Maynard, C.A. (2007) Restoration of threatened species: A noble cause for transgenic trees. Tree Genetics & Genomes 3: 111-118
Rothrock, R.E., Polin-Mcguigan, L.D., Newhouse, A.E., Powell, W.A. and Maynard, C.A. (2007) Plate flooding as an alternative Agrobacterium-mediated transformation method for American chestnut somatic embryos. Plant Cell, Tissue and Organ Culture 88: 93-99
Welch, A.J., A.J. Stipanovic, C.A. Maynard, and W.A. Powell. (2007) The effects of oxalic acid on transgenic Castanea dentata callus tissue expressing oxalate oxidase. Plant Science 172:488-496.

 

Back to top

U.S. Patent

Title: Antimicrobial Peptides

Covers genes encoding antimicrobial peptides designed for enhancing pathogen resistance in plants.

U.S. Patent No. 5,856,127

Date of Patent: 1/5/99

Abstract: The present invention is directed to antimicrobial polypeptides and to nucleic acid molecules encoding the antimicrobial polypeptides. The polypeptide consists of 15 to 20 amino acids and has an amphipathic alpha helix structure, wherein 3 or more of the amino acids form a positively charged domain extending axially along the alpha helix. Expression vectors, host cells, and transgenic plants, as well as methods of producing plants having improved resistance to fungal and bacterial infestation, are also proved.

For full text click here