ERE 596 Microtechnique
Spring Semester 2005
Instructor: S. E. Anagnost
Tuesday 9:00 - 12:00
Topics to be covered:
Knife sharpening and care.
Use of the sliding microtome.
Block preparation (trimming, softening) for sectioning.
Block orientation in the sliding microtome.
Staining of sections with hematoxylin and safranin.
Section dehydration and slide preparation.
Fiber suspension preparation.
1. Slides of 4 wood species (oak, Quercus alba; red maple, Acer rubrum; white pine, Pinus strobus and southern yellow pine, Pinus palustris) stained with hematoxylin and safranin and mounted in Permount. Each slide will have a radial, tangential and transverse section. Sections must show proper orientation, be free of knife marks and tears. Section edges should be trimmed with a fresh razor blade.
2. Two slides of fibers prepared from macerated wood. One slide should be prepared of a hardwood (oak) and one of a softwood (maple). Use Franklin's method to pulp wood into fibers.
The slides are due at the end of the semester.
ERE 596 Section 19 Microtechnique
February 1 Knife sharpening and care; use of sliding microtome
Reading assignment: Microtechnique handout, p. 2-7
Keith and C™tˇ, 1968
Kennedy and Chan, 1970.
February 8 Block preparation; sliding microtome operation
Prepare radial, tangential and transverse sections of white pine
February 15 Continue to cut sections of white pine
Reading assignment: Microtechnique handout, p. 8-12
February 22 Critique of white pine sections
Staining protocols and slide preparation
Stain and prepare a slide of white pine sections
March 1 Critique of white pine slides
Start preparing blocks and sectioning a diffuse porous hardwood (maple), ring porous hardwood (oak), and southern yellow pine.
Reading assignment: Kukachka, 1977; Fujita and Harada, 19??.
March 8 Continue sectioning; block softening techniques
March 15 No classŃSpring Break
March 22 Continue with sectioning, staining and slide preparation
March 29 Continue with sectioning, staining and slide preparation
Reading assignment: Microtechnique handout, p. 13-14
April 5 Pulp wood (oak and pine) into fibers using Franklin's method
April 12 Slide preparation of fibers of two species
April 19 Continue with sectioning, staining and slide preparation
(no class this week)
April 26 Critique of fiber slides
May 2 Slides due (4 wood species and 2 fiber preparations).
Microtechnique handout Pages 5 - 16.
Berlyn, G.P. and J.P. Miksche. 1976. Botanical Microtechnique and Cytochemistry. The Iowa State University Press, Ames, Iowa. 326 pp.
Fujita, M. and H. Harada. 19??. A simple embedding and lining method for the sectioning of wood and wood-based materials using an alpha-cyanoacrylate resin.
Keith, C.T. and W. A. C™tˇ. 1968. Microscopic characterization of slip lines and compression failures in wood cell walls. For. Prod. J. 18(3):67-74.
Kennedy, R.W. and C.K. Chan. 1970. Tensile properties of microsections prepared by different microtomy techniques. 5(1):39-42.
Kukachka, B.F. 1977. Sectioning Refractory Woods for Anatomical Study. USDA Forest Service Research Note FPL-0236, USDA Forest Service, Forest Products Laboratory, Madison WI. 9 pp.