The N.C. Brown Center for Ultrastructure Studies is the central microscopy facility at SUNY-ESF, located on the second floor of Baker Lab. The Center for Ultrastructure Studies provides students, faculty and research staff with access, assistance, and training in modern microscopy techniques. These techniques include light microscopy, scanning electron microscopy, transmission electron microscopy, digital imaging, image analysis and a host of ancillary applications.
This facility was first established in 1957 with the installation of the first transmission electron microscope in central New York. The N.C. Brown Center for Ultrastructure Studies was formally established in 1972 when anETEC scanning electron microscope was installed in Baker Laboratory. The Center has always maintained a position of leadership and excellence in the areas of wood structure and microscopy, both nationally and internationally.
Many departments and programs within the College and neighboring universities are represented among the faculty, staff and students who utilize the Center for research. During an average year, 30 faculty, 35 graduate students, and numerous undergraduate students utilize the resources of the Center for their research. Outreach activity is also an important service provided by the Center. These services include providing micrographs for educational purposes, assistance to other universities, tours and demonstrations to interested technology groups, and microscopy assistance to private industry. The types of private industries for which we provided microscopy services include forest product industries, pulp and paper manufacturers, wood preservation companies, consulting engineering firms, and large pharmaceutical companies.
For information contact 470-6637. The SALTS lab is ELAP certified for Phase Contrast Microscopy fiber analysis (NIOSH 7400 A RULES). The Environmental Laboratory Approval Program (ELAP) is part of the Wadsworth Center, New York State Department of Health.
Our current scanning electron microscope is a JEOL JSM-5800 LV low vacuum scanning electron microscope equipped with an EDAX energy dispersive x-ray spectrometer. The JSM 5800 LV has superior resolution in high vacuum mode, a large chamber for microtesting, and a motorized eucentric stage for unattended analysis. The instrument will operate in the conventional high vacuum mode or the low vacuum mode of operation.
The JSM 5800 LV is equipped with an EDAX x-ray microanalysis system. The energy dispersive spectrometer (EDS) is an essential and integral component of any multidisciplinary scanning electron microscopy facility. EDS x-ray analysis is employed for the identification and localization of the chemical components within the specimen.The lab is equipped with a Tousimis PVT-3B critical point dryer and with metal and carbon coaters for superior specimenresolution.
Our transmission electron microscope is a JEOL JSM-2000EX. The JSM 2000EX has an accelerating voltage of 80-200 kV. This instrument has a lattice image resolution of 0.14nm and a point image resolution of 0.28 nm and can be operated at a magnification of 1,000,000 X. The variable accelerating voltage permits the observation of negative stained proteins, bacteria, viruses or ultrathin sections at the lower kV as well as superior resolution of nanoparticles and thick specimens at higher voltages. It has a side entry tilt stage goniometer that can perform 3D reconstruction of macromolecules, single particles and tomography of whole cells. It can also perform micro electron diffraction yielding atomic resolution on thin samples as has been accomplished with purple membrane. Other capabilities are a specimen position memory function, an auto through focus function, an optimum underfocus function, a minimum dose system, and an image data recording function. The lab has ancillary equipment that allows for ultrathin (60nm) sectioning using diamond knives of resin or frozen material, freeze-substitution, rotary shadowing, immunolabeling, freeze fracturing of various samples both biological and materials.
The Center is outfitted with an array of Zeiss and Nikon light microscopes that are equipped for various LM techniques. These techniques include brightfield (transmitted and reflected), phase contrast, darkfield, UV fluorescence using multiple filters, video enhanced contrast, polarization, oil immersion, and Nomarski differential interference contrast. Images are recorded using a Spot RT or K digital cameras (1600x1200 pixels) and recorded into Spot, Image Pro Plus 7.0, Image J or Photoshop. The lab is set up for correlative microscopy where the same sample is viewed by light, scanning and transmission electron microscopy. The lab has several sliding microtomes for preparing thin sections of wood or hardened materials for light microscopy.
The Academic program offered by the Center consists of graduate and undergraduate courses, graduate level special topic research projects, and graduate student guidance. We offer a twelve-credit minor in microscopy for undergraduates. Our program is unique in central New York. We are the only one offering comprehensive formal training in the theory and application of these research tools. The courses offered are:
Graduate/ Advanced Undergraduate
Microscopy Minor (12 credits):
Due to the special nature of these courses, the optimal enrollment where ‘hands-on’ practical microscope use is involved is 8-10 students.
MCR 480/680 is an undergraduate or graduate level survey course that touches on theory of all microscopes used today such as atomic force, Raman, Near Field as well as techniques employed such as In-situ hybridization, autoradiography and Immuno-gold labeling. Also covered is the cellular structure and substructure encountered during tissue examination using light and electron microscopes.
(Students registered for an ESF MCR courses are not included)*
|Transmission Electron Microscope (TEM) (JEOL 2000EX)/hr||$40||50||100|
|High Resolution TEM 120kV and higher/hr||50||60||120|
|Scanning Electron Microscope (SEM) (JEOL 5800LV)/hr||40||50||100|
|Freeze Fracture Techniques (Balzers 400T)/hr||40||50||100|
|Cryotechniques (Ultramicrotomy;Freeze Substitution) (UC6)/hr||40||50||100|
|Resin Ultramicrotomy (hydrophobic or Hydrophilic)/sample||40||50||100|
|Usage Charges: Biological/Materials|
|Light microscopy (BF,PC,Nomarski, PL)/slide||10||15||30|
|Stained semi-thin sections/slide (TEM)||10||15||30|
|Fluorescence sample prep/slide||40||50||100|
|Critical Point Drying/run||30||45||90|
|Sputter Coating Au/Pd/run||20||30||60|
|SEM elemental analysis/sample||40||50||100|
|High Resolution TEM (120kV and higher)/hr||50||60||120|
|Tissue processing and embedding in plastic/4 blocks||60||90||180|
|Autoradiography (tritium, iodine 125)/sample||500||1000||2000|
|Immunoelectron microscopy IEM (LM,SEM or TEM)/sample||500||1000||2000|
|Wet photography and film scanning/each||2||3||6|
|Freeze Fracture/sample (with IEM add $500)||500||1000||2000|
|Negative staining (UA,PTA,Va,Mo)/grid||10||15||30|
|Airfuge particle count (includes negative stain TEM)/run||500||1000||2000|
|Rotary shadow (Pt,Au,Cr,Ti,Ta)/run||50||60||120|
|Electron tomography and 3D reconstruction/grid||500||1000||2000|
* Students may be charged lab fee for certain courses
** Industry Rate is normally 2X Outside Academia Rate unless otherwise noted
The N.C. Brown Center for Ultrastructure Studies at SUNY-ESF must be acknowledged in all publications that include data derived from the Center. Include this statement: "Microscopy was performed at the N.C. Brown Center for Ultrastructure Studies at SUNY-ESF, Syracuse, NY."
If N.C. Brown personnel performed the work for you they should be acknowledged by name.
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Updated: June 2013