Danilo Fernando

Danilo D. Fernando
Associate Professor

461 Illick Hall
1 Forestry Dr.
Syracuse, New York 13210

Phone: (315) 470-6790


Research Interests

Research in my lab focuses on the reproductive development of plants, particularly woody species such as pine, willow and American chestnut. We are interested in elucidating the mechanisms involved in pollen germination, pollen tube wall formation, gamete formation, and reproductive sterility using a combination of approaches involving anatomy, cell biology, genetics, genomics and proteomics. We are also interested in the evolution of reproductive structures in seed plants and manipulations of reproductive structures for genetic improvement and biotechnology.


  • Mechanism of pollen tube development in conifers involving microarray, microRNA profiling, and high-throughput proteomics.
  • Willow flowering and wood development involving histology, genomics, and bioinformatics; willow in vitro regeneration and genetic transformation for functional analysis and biotechnology.
  • Genetic transformation of American chestnut pollen; establishing range of viable pollen dispersal.
  • Analysis of genetic diversity in rare and threatened ferns using molecular markers; facilitating cross-fertilization through in vitro co-cultivation of fern gametophytes.

Courses Taught

BTC 132: Biotechnology Orientation Seminar

Course description and objectives: This course is designed for incoming undergraduate students (freshmen and transfers). The students will be introduced to various campus resources, different research disciplines in biotechnology, and faculty members and students in the program. At the end of the course, the students will gain insights useful for course selection and future career choices, appreciate the interconnections amongst the various disciplines, and develop professional skills and attitudes. This course will also serve as a forum to address issues that typically do not fit well into the traditional courses.

Course requirements: Regular attendance to lectures, participation in field trips/tours, and class discussions.


Two hours of lecture and one three-hour laboratory. An evolutionary survey of plants from unicellular prokaryotes to multicellular eukaryotes. Coverage includes the algae, fungi, bryophytes, lycopods, ferns, fossil plants, gymnosperms and angiosperms. 3 credits, Spring.

Don't miss the EFB 326 course web page, featuring videos, flashcards, answers to text questions-- it's way cool!:


Two hours of lecture and three hours of laboratory. Advances in the fields of genetics and molecular biology are integrated into a dynamic study of plant structure and development. Topics include fertilization, embryogenesis, gene expression and manipulation, and hormonal and environmental regulation of development. 3 credits, Fall.


One hour of presentations and discussion. Sexual reproduction in woody species and its importanct to breeding, conservation, and biotechnology are emphasized. 1 credit, Fall.

Recent Publications

Quinn CR, Iriyama R, and Fernando DD. 2014. Computational Predictions and Expression Patterns of  Conserved MicroRNAs in Loblolly Pine (Pinus taeda). Tree Genetics & Genomes (in press).

Fernando DD. 2014. The pine reproductive process in temperate and tropical regions. New Forests 45: 333-352.

Quinn CR, Iriyama R, and Fernando DD. 2014. Expression of conserved microRNAs in the male gametophyte of loblolly pine. Plant Reproduction 27: 69-78.

Seago JL and Fernando DD. 2013. Anatomical aspects of angiosperm root evolution. Annals of Botany 112 (2): 1-16.

Fernando DD, Quinn CR, Brenner E, and Owens JN. 2010. Male gametophyte development and evolution in gymnosperms. Intl J Plant Developmental Biology 4 (Special Issue 1): 47-63. PDF

Fernando DD, Zhang S. 2008. Characterization and heterologous expression of SLF, a functional homolog of the floral regulator LEAFY/FLORICAULA from Salix discolor. International Journal of Plant Developmental Biology 2: 92-99. PDF

Wang BY, Su JR, Fernando DD. 2008. Development of the male reproductive structures in Taxus yunnanensis. Plant Systematics and Evolution 276: 51-58. PDF

Owens JN and Fernando DD. 2007. Pollination and seed production in western white pine. Canadian Journal of Forest Research 37: 260-275.

Fernando DD, Richards JL and Kikkert JR. 2006. In vitro germination and transient GFP expression of American chestnut (Castanea dentata) pollen. Plant Cell Rep 25: 450-456. PDF

Fernando DD. 2005. Characterization of pollen tube development in eastern white pine (Pinus strobus) through proteomic analysis of differentially expressed proteins. Proteomics 5: 4917-4926. PDF

Fernando DD and Zhang S. 2005. Constitutive expression of the SAP1 gene from willow (Salix discolor) causes early flowering in Arabidopsis thaliana. Development, Genes and Evolution 14: 1-10. PDF

Fernando DD, Lazzaro MD and Owens JN. 2005. Growth and development of conifer pollen tubes. Sexual Plant Reproduction 18: 149-162. PDF

Fernando DD, Long SM and Sniezko RA. 2005. Sexual reproduction and crossing barriers in white pines: the case between Pinus lambertiana (sugar pine) and P. monticola (western white pine). Tree Genetics and Genomes 1: 143-150. PDF

Zhang S and Fernando DD. 2005. Structural, histochemical and protein analysis of male reproductive development in willow. Sexual Plant Reproduction 18: 37-41. PDF

Older Publications

Fernando DD and Owens JN. 2004. Development of an in vitro technology to confer white pine blister rust resistance. In Sniezko, R.A. et al. eds. 2004. Breeding and genetic resources of five-needle pines: growth, adaptability and pest resistance; 2001 July 23-27; Medford, OR, USA. IUFRO Working Party 2.02.15. Proceedings RMRS-P-32. Fort Collins, CO: US Department of Agriculture, Forest Service, Rocky Mountain Research Station. Pp. 163-168.

Mueller JK, Heckathorn SA and Fernando DD. 2003. Identification of a chloroplast dehydrin in leaves of mature plants. International Journal of Plant Sciences 164: 535-542.

Owens JN, Takaso T and Fernando DD. 2003. Conifer reproduction: diversity in an ancient group. Acta Horticulturae 615: 115-120.

Fernando DD, Owens JN, Yu X, and Ekramoddoullah AKM. 2001. RNA and protein synthesis during in vitro pollen germination and tube elongation in Pinus monticola and other conifers. Sexual Plant Reproduction 13: 259-264.

Fernando DD, Owens JN and Misra S. 2000. Transient gene expression in pine pollen tubes following particle bombardment. Plant Cell Reports 19: 224-228.

Fernando DD, Owens JN and von Aderkas P. 1998. In vitro fertilization from co-cultured pollen tubes and female gametophytes of Douglas fir (Pseudotsuga menziesii). Theoretical and Applied Genetics 96: 1057-1063.

Fernando DD, Owens JN, von Aderkas P and Takaso T. 1997. In vitro pollen tube growth and penetration of female gametophyte in Douglas fir (Pseudotsuga menziesii). Sexual Plant Reproduction 10: 209-216.

Fernando DD and Cass DD. 1997. Developmental assessment of sexual reproduction in Butomus umbellatus (Butomaceae): male reproductive component. Annals of Botany 80: 449-456.

Fernando DD and Cass DD. 1997. Developmental assessment of sexual reproduction in Butomus umbellatus (Butomaceae): female reproductive component. Annals of Botany 80: 457-467.

Fernando DD and Cass DD. 1996. Genotypic differentiation in Butomus umbellatus (Butomaceae) using isozymes and random amplified polymorphic DNAs. Canadian Journal of Botany 74: 647-652.

Fernando DD and Cass DD. 1996. Development and structure of ovule, embryo sac, embryo, and endosperm in Butomus umbellatus (Butomaceae). International Journal of Plant Sciences 157: 269-279.

Fernando DD and Cass DD. 1994. Plasmodial tapetum and pollen wall development in Butomus umbellatus (Butomaceae). American Journal of Botany 81: 1592-1600.

Current Graduate Advisees

Current Graduate Advisees

Rie IriyamaRie Iriyama

  • Degree Sought: MS
  • Graduate Advisor(s): Fernando
  • Area of Study: Plant Science & Biotechnology

Jennifer PotrikusJennifer Potrikus

  • Degree Sought: MS
  • Graduate Advisor(s): Fernando
  • Area of Study: Conservation Biology

Michael ServissMichael Serviss

  • Degree Sought: MS
  • Graduate Advisor(s): Fernando
  • Area of Study: Conservation Biology

State University of New York College of Environmental Science and Forestry
1 Forestry Drive | Syracuse, NY 13210 | 315-470-6500
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