State University of New York

College of Environmental Science and Forestry

Faculty of Forestry


Charles A. Maynard
Faculty of Forestry
State University of New York
College of Environmental Science and Forestry
Syracuse, New York 13210
(315) 470-6560
Fax: (315)470-6956
cmaynard@mailbox.syr.edu.



Welcome to my web page, you are visitor number .


You're missing a stunning portrate of Dr. Maynard ;)

Dr. Maynard examining a black cherry tissue culture plantlet flowering
after only the second growing season in the field.
The photo was taken in about 1985.
The subject on the left has grown considerably,
and the subject on the right has defoliated somewhat .

keywords: agricultural biotech biotechnology biochemistry botany castanea chestnut black cherry prunus willow salix chestnut blight cryphonectria plant disease environment forest genetics Forestry gene genetic engineering hardwood molecular biology transformation poplar populus phytopathology recombinant DNA seed orchards septoria tissue culture micropropagation transgenic tree improvement

Education
Iowa State UniversityForest Biology & Wood Science 1980 Ph.D.
Iowa State University Forest Biology1977 M.S.
Iowa State University Forest Management1974 B.S.


Professional Experience

1980 to presentFull professor (1994), Associate Professor (1986-1994), Assistant Professor (1983-1986), and Research Associate (1980-1983), forest genetics and tree improvement. SUNY/ESF Faculty of Forestry.
1977-1980Research Assistant, forest genetics. Iowa State University, Forestry Department.
1975-1977Forestry Extension Assistant. Iowa State University, Forestry Department.
1974Field Foreman. Western Maine Forest Nursery Co., Fryeburg, Maine.

Courses Taught

Refereed Journal Publications

Book Chapters

Conference Papers & Presentations

Research Accomplishments

Recent Writings From Our Lab

Forest Genetics Glossary

Chestnut Embryogenesis (Abstract)

Black Cherry Field Test

American Chestnut Genetic Engineering Project

PCR for Identifying Transgenic Plants

Research Accomplishments

Forest tree improvement

In the early 1980s, I worked closely with the New York State Department of Environmental Conservation, Division of Lands and Forests on their applied forest genetics program. One of my major accomplishments for DEC was to write and begin implementation of a 10-year plan, outlining goals and strategies for tree improvement by the State of New York (Maynard 1983b). Priorities were set among the different species. Existing provenance tests were remeasured (Easley and Maynard 1986, 1987; Maynard 1983a). Pesticides were tested for controlling seed orchard insects (Valenti, Abrahamson, and Maynard 1990). Seed was collected from each tree in three Norway spruce (Picea abies) seed orchards and Norway spruce "plus trees." In all, nearly 150 Norway spruce seedlots were established in field tests.


Tissue culture of high-value hardwoods

Concurrent with my forest genetics activities, I was working with a series of graduate students on developing tissue culture propagation methods for black cherry (Prunus serotina). This project led to a number of important accomplishments. We were the first to establish, multiply, and root black cherry in vitro (Tricoli, Maynard, and Drew 1985), the first to use gibberellin to break dormancy in a forest tree species - it had been used several times on woody horticultural crops (Kavanagh, Maynard, and Drew 1987; Drew, Kavanagh, and Maynard 1988), and the first to identify an enhancing effect of dimethylsulfoxide (DMSO) on bud break (Kavanagh, Lee, Drew, and Maynard 1993). We were also one of the first research groups to identify an inhibiting effect of blue light on root formation (Fuernkranz, Nowak, and Maynard 1990). Our nine-year-old stand on Heiberg Forest is the oldest field planting of tissue culture propagated black cherry in the country (Maynard 1994). The project, through initial field results, was summarized in a book chapter (Maynard, Kavanagh, Fuernkranz, and Drew 1991).

Genetic engineering

In 1988, I began working with American chestnut (Castanea dentata), first on pollen collection and storage techniques (Maynard 1991d), then in collaboration with Dr. William Powell, on engineering blight resistance into American chestnut. I have transformed a selectable-marker gene into chestnut callus tissue and confirmed transformation using the polymerase chain reaction (PCR) technique (Maynard 1991a). My graduate students and I have also been working on embryogenesis from immature ovules, as well as rooting and acclimatization techniques (Maynard, Satchwell, and Rieckermann 1993). Dr. Powell has developed a small polypeptide that is capable of killing fungal spores in vitro. We tested it on chestnut, willow, apple and petunia tissue in vitro and found it to be non-phytotoxic. Dr. Powell had the gene synthesized and we are testing it along with several others for correct expression and toxicity in vivo Results to date indicate that we have successfully transferred the gene into poplar clone "Ogy."