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Analytical and Technical Services
Nuclear Magnetic Resonance (NMR) Spectrometry

NMR spectroscopy is among the most powerful analytical techniques for the elucidation of chemical structure. It has the capability of providing detailed information about structure and dynamics of molecules as well as how atoms are connected to each other. NMR can identify different nuclei in a molecule and analyze large proteins and other large molecules using three dimensional (3-D) techniques. This instrument provides powerful information for the researcher to identify compounds, study the structure-function relationship of atoms or the molecules in which they are contained.

The college currently operates two Bruker NMR spectrometers: an AVANCE III HD 800 MHz and an AVANCE III 600 MHz. All instruments are capable of multi-dimensional NMR experiments and are non-destructive to the sample.

Bruker 800 MHz Spectrometer

The Bruker 800 MHz Spectrometer utilizes an 18.8 Tesla superconducting magnet and has a sample temperature range of -40 to 80 C. The system has a Sample Transport autosampler that allows up to 24 samples to be run unattended. This NMR spectrometer is ultrasensitive and equipped with four channels with gradients and has the capability of analyzing liquid samples.

Probes

“TCI Cryo Probe is a proton-optimized triple resonance NMR 'inverse' probe, featuring three fully independent channels (plus lock channel) for simultaneous decoupling on multiple nuclei such as 1H, 2H, 13C and 15N.

Triple Resonance Probes give you access to advanced NMR techniques, letting you pulse up to four nuclei in one experiment. The TCI Cryo Probe is optimized for 1H and 13C observation. With the deuterium lock switch, you can decouple 2H, as well, permitting decoupling of up to four nuclei in a single pulse sequence. Triple (TCI) Resonance Probes are used extensively for the family of double and triple resonance experiments developed to perform NMR structure determination of biological macromolecules.”

This is a shared resource with the following Universities: Upstate Medical, SUNY ESF, Cornell, Rochester, and Buffalo. The instrument was purchased with a grant from NIH of $2M.

Bruker 600 MHz Spectrometer

The Bruker 600 MHz Spectrometer utilizes a 14.1 Tesla superconducting magnet and has a sample temperature range of -70 to 150 C. The system has a Sample Transport autosampler that allows for up to 24 samples to be run unattended. This NMR spectrometer is ultrasensitive and equipped with 3 channels with gradients and has the capability of analyzing solid, liquid and intermediate (gel) samples.

Probes

Liquid State:

  • Prodigy 5 mm BBFO liquid nitrogen cold probe.
  • sensitivity boost of X Nuclei by a factor of 2-3
  • Broadband technology covering nuclei from15N to 31P, plus 19F
  • SmartProbe- BBFO (Broad Band Fluorine Observation) with single axis gradients.  The probe automatically tunes for all nuclei and has the highest sensitivity on both multinuclear and proton channel.
  • 1mm TXI 1H/13C/15N  a triple resonance probe with single axis gradients. Ultimate sensitivity for the smallest of samples. The probe only needs ~ 25ng of material, Homonuclear 2D spectra can be acquired with ~ 250ng, heteronuclear with ~ 2ug of sample.

Solids/Gel samples:

  • HRMAS/CPMAS (Doty) 4mm rotor size probe that can handle samples that are either crystalline or amorphous materials. 

Tuning Options:
1H/X/Y - Double-broadband X & Y channels
1H/X - broadband X channel (limited range)

NMR Rates