SEARCH:

Nuclear Magnetic Resonance (NMR) Spectrometry

Dave Kiemle standing near a NMR machine
Dave Kiemle, Instructional Support Specialist
B-18 Jahn Laboratory
470-6883
dkiemle@syr.edu

NMR spectroscopy is among the most powerful analytical techniques for the elucidation of chemical structure.  It has the capability of providing detailed information about structure and dynamics of molecules as well as how atoms are connected to each other.   NMR can identify different nuclei in a molecule and analyze large proteins and other large molecules using three dimensional (3-D) techniques.  This instrument provides powerful information for the researcher to identify compounds, study the structure-function relationship of atoms or the molecules in which they are contained.

The college currently operates three Bruker NMR spectrometers: an AVANCE III HD 800 MHz, an AVANCE III 600 MHz and an AVANCE 300 MHz.  All instruments are capable of multi-dimensional NMR experiments and are non-destructive to the sample.

The Bruker 800 MHz Spectrometer utilizes an 18.8 Tesla superconducting magnet and has a sample temperature range of -40 to 80 C. The system has a Sample Transport autosampler that allows up to 24 samples to be run unattended. This NMR spectrometer is ultrasensitive and equipped with four channels with gradients and has the capability of analyzing liquid samples.

Probes

“TCI Cryo Probe is a proton-optimized triple resonance NMR ‘inverse’ probe, featuring three fully independent channels (plus lock channel) for simultaneous decoupling on multiple nuclei such as 1H, 2H, 13C and 15N.

Triple Resonance Probes give you access to advanced NMR techniques, letting you pulse up to four nuclei in one experiment. The TCI Cryo Probe is optimized for 1H and 13C observation. With the deuterium lock switch, you can decouple 2H, as well, permitting decoupling of up to four nuclei in a single pulse sequence. Triple (TCI) Resonance Probes are used extensively for the family of double and triple resonance experiments developed to perform NMR structure determination of biological macromolecules.”

This is a shared resource with the following Universities: Upstate Medical, SUNY ESF, Cornell, Rochester, and Buffalo.  The instrument was purchased with a grant from NIH of $2M.

Usage Fees:

Minimum of 1/2 hour charge for usage with all groups below:

Sponsored Research - independently: $8 per hour, ATS staff support: $8 per hour
Instruction/Non-Sponsored - independently: $8 per hour, ATS staff support: $8 per hour
External Partner Use (agreement required) - $8 per hour
External Academic Use - $15 per hour
External Private Use - operator charge of (at cost) plus $50 per hour for instrument

We typically analyze prepared samples but can train appropriate users to operate the equipment.  Assistance on experiment design and interpretation of results is also available.  The instrument is scheduled via Faces online scheduling system, email Dave Kiemle for password and logon account.

The Bruker 600 MHz Spectrometer utilizes a 14.1 Tesla superconducting magnet and has a sample temperature range of -70 to 150 C. The system has a Sample Transport autosampler that allows for up to 24 samples to be run unattended. This NMR spectrometer is ultrasensitive and equipped with 3 channels with gradients and has the capability of analyzing solid, liquid and intermediate (gel) samples.

Probes

Liquid State:

  • Prodigy 5 mm BBFO liquid nitrogen cold probe.
    • sensitivity boost of X Nuclei by a factor of 2-3
    • Broadband technology covering nuclei from15N to 31P, plus 19F
  • SmartProbe- BBFO (Broad Band Fluorine Observation) with single axis gradients.  The probe automatically tunes for all nuclei and has the highest sensitivity on both multinuclear and proton channel.
  • 1mm TXI 1H/13C/15N  a triple resonance probe with single axis gradients. Ultimate sensitivity for the smallest of samples. The probe only needs ~ 25ng of material, Homonuclear 2D spectra can be acquired with ~ 250ng, heteronuclear with ~ 2ug of sample.

Solids/Gel samples:

  • HRMAS/CPMAS (Doty) 4mm rotor size probe that can handle samples that are either crystalline or amorphous materials. 

Tuning Options:
1H/X/Y - Double-broadband X & Y channels
1H/X - broadband X channel (limited range)

Usage Fees:

Minimum of 1/4 hour per sample with all groups below:

Sponsored Research - independently: $8 per hour, ATS staff support:
Instruction/Non-Sponsored - independently: $8 per hour, ATS staff support:
External Partner Use (agreement required) - $10 per
External Academic Use - $15 per hour
External Private Use - operator charge of (at cost) plus $30 per hour for instrument

We typically analyze prepared samples but can train appropriate users to operate the equipment.  Assistance on experiment design and interpretation of results is also available.  The instrument is scheduled via email to Dave Kiemle.

The 300 MHz instrument is a walk-on system and students are encouraged to fully operate the equipment. The system has a NMR CASE Autosampler that allows for up to 25 samples to be run unattended. It also has an Autosampler for the solids that can run up to 20 samples.

Probes:

Liquid state - BBFO Broad banded Observe with automatic tuning for all nuclei including 19F.

Solid State -7mm Broad Band CPMAS solid state probe. Spinning speed of 7000 Hz, and a new 7mm Broad Band CPMAS solid state probe. Spinning speed of 9000 Hz

Usage Fees:

Minimum of 1/4 hour per sample with all groups below ($2.00 per sample):

Sponsored Research - independently: $4 per hour, ATS staff support: $4 per hour
Instruction/Non-Sponsored - independently: $4 per hour, ATS staff support: $4 per hour
External Partner Use (agreement required) - $6 per hour
External Academic Use - $6 per hour
External Private Use - operator charge of (at cost) plus $15 per hour for instrument
Solid State NMR at minimum charge of $25.00 per sample, at @ $8.00 per hour.

Contact: Dave Kiemle at dkiemle@esf.edu or 470-6883/6846


Social

YouTube Channels

ESF | Athletics | Research | Academics | Sustainability | New York | Nature


SUNY-ESF
State University of New York College of Environmental Science and Forestry
SUNY-ESF |
1 Forestry Drive | Syracuse, NY 13210 | 315-470-6500
Copyright © 2016 | Admissions Information | Webmaster